This function performs doublet-calling using the scrublet(python) package on a Seurat object.
Usage
db_Scrublet(srt, assay = "RNA", db_rate = ncol(srt)/1000 * 0.01, ...)Arguments
- srt
A Seurat object.
- assay
The name of the assay to be used for doublet-calling. Default is "RNA".
- db_rate
The expected doublet rate. Default is calculated as ncol(srt) / 1000 * 0.01.
- ...
Additional arguments to be passed to scrublet.Scrublet function.
Examples
data("pancreas_sub")
pancreas_sub <- db_Scrublet(pancreas_sub)
#> Try to install scrublet ...
CellDimPlot(pancreas_sub, reduction = "umap", group.by = "db.Scrublet_class")
FeatureDimPlot(pancreas_sub, reduction = "umap", features = "db.Scrublet_score")
