This function performs doublet-calling using the scDblFinder package on a Seurat object.
Usage
db_scDblFinder(srt, assay = "RNA", db_rate = ncol(srt)/1000 * 0.01, ...)
Arguments
- srt
A Seurat object.
- assay
The name of the assay to be used for doublet-calling. Default is "RNA".
- db_rate
The expected doublet rate. Default is calculated as ncol(srt) / 1000 * 0.01.
- ...
Additional arguments to be passed to scDblFinder::scDblFinder function.
Examples
data("pancreas_sub")
pancreas_sub <- db_scDblFinder(pancreas_sub)
CellDimPlot(pancreas_sub, reduction = "umap", group.by = "db.scDblFinder_class")
FeatureDimPlot(pancreas_sub, reduction = "umap", features = "db.scDblFinder_score")